Recently Published Research by IPAS member Mel Mcdowall
Redox and anti-oxidant state within cattle oocytes following in vitro maturation with bone morphogenetic protein 15 and follicle stimulating hormone
We demonstrated that the metabolic activity of cattle oocytes (eggs) varies in response to different hormones and growth factors, namely follicle stimulating hormone (FSH) and bone morphogenetic protein 15 (BMP15), two common additives used to culture oocytes in vitro. Specifically, BMP15 influences the activity and characteristics of mitochondria (the power stations of the cell) in a manner that is associated with improved health of oocytes and subsequent embryos.
This study is unique in that custom made, fluorescence markers of metabolism, plus autofluorescence (the natural glow of cells, hence no chemicals were used to create the signal) were used to not only look at the total activity of the cells, but the localisation of activity and patterns of expression, with in live cells. Most techniques involve toxic labelling or the cells to be fixed.
This work is a collaboration with The University of Melbourne, Macquarie University, University of New South Wales and University of Buenos Aires, Argentina.
Authors: Sutton-Mcdowall, M.L., Purdey, M., Brown, H.M., Abell, A.D., Mottershead, D.G., Cetica, P.D., Dalvit, G.C., Goldys, E.M., Gilchrist, R.B., Gardner, D.K., Thompson, J.G.
Molecular Reproduction and Development 82 (4) 281–294 (2015)
Posted on July 3, 2015, in IPASnews, Paper Summaries and tagged anti-oxidant, institute for photonics and advanced sensing, ipas, melanie mcdowall, Redox, the university of adelaide, university of adelaide. Bookmark the permalink. Leave a comment.